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wizard sv gel and pcr clean-up system purification kit  (Promega)

 
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    Structured Review

    Promega wizard sv gel and pcr clean-up system purification kit
    Wizard Sv Gel And Pcr Clean Up System Purification Kit, supplied by Promega, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/wizard+sv+gel+and+pcr+clean-up+kit/wizard+genomic+dna+purification+kit/pmc12280279-78-8-7
    Average 90 stars, based on 1 article reviews
    wizard sv gel and pcr clean-up system purification kit - by Bioz Stars, 2026-09
    90/100 stars

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    Related Articles

    Polymerase Chain Reaction:

    Article Title: Barcoded Rabies In Situ Connectomics for high-throughput reconstruction of neural circuits
    Article Snippet: .. To size-select the resultant linear products, the PCR reaction was loaded on an agarose gel, and gel extraction was performed with a Wizard SV Gel and PCR Clean-Up kit (Promega, A9281). .. Secondary purification was then carried out with DNA Clean & Concentrator-5 columns (Zymo Research, D4004).

    Article Title: Barcoded Rabies In Situ Connectomics for high-throughput reconstruction of neural circuits
    Article Snippet: .. The UMI-tagged viral amplicons were first gel-purified with a Wizard SV Gel and PCR Clean-Up kit (Promega, A9281) and eluted with 20 μl water. ..

    Article Title: Barcoded Rabies In Situ Connectomics for high-throughput reconstruction of neural circuits
    Article Snippet: Thermocycling conditions were likewise the same as the Illumina adaptor PCR, but with a fixed number of 3 cycles. .. The indexed viral and plasmid amplicons were then gel-purified with a Wizard SV Gel and PCR Clean-Up kit (Promega, A9281) and eluted with 25 μl water. .. The concentration and size distribution of these amplicon libraries were quantified with a High Sensitivity D1000 ScreenTape kit (Agilent, 5067-5584) on an Agilent 2200 TapeStation system.

    Article Title: The extracellular region of Trypanosoma congolense Membrane Bound Acid Phosphatase induces partial protection in immunized BALB/c mice.
    Article Snippet: African trypanosomiasis is a parasitic disease caused by African trypanosomes and there is no vaccine because the trypanosomes possess an intricate machinery for antigenic variation.. Owing to the nonsusceptibility of trypanosomes’ Membrane-Bound Acid Phosphatase (MBAP; EC 3.1.3.2) to antigenic variation and its central role in molecular trafficking, we hypothesized that MBAP is a promising target for vaccine development.. Herein, a DNA vaccine was constructed using recombinant DNA technology to insert the gene of the extracellular portion (EP) of Trypanosoma congolense MBAP without its signal peptide (SP), into a Strep-tag/transin modified mammalian expression vector pVAX1.

    Article Title: Metagenomic and meta-metabolomic analysis of traditional Korean rice vinegar productions shows a large variability between producers.
    Article Snippet: For the fungal communities, the internal transcribed spacer 1 (ITS1) region of the rRNA transcribed unit was amplified by PCR using the primers BITS/B58S3 (Bokulich and Mills, 2013), extended with an Illumina-specific 5′ tag, as described previously (Vermote et al., 2018). .. The V4 and ITS1 amplicons obtained were purified using a Wizard SV gel and PCR clean-up kit (Promega, Madison, WI, USA) and size selected using AMPure XP magnetic beads (Beckman Coulter, Brea, CA, USA), according to the manufacturers’ instructions. .. The amplicon size distribution was determined with capillary electrophoresis using a Bioanalyzer 2100 with a High Sensitivity DNA Kit (Agilent, Santa Clara, CA, USA), and the concentrations were measured with a Qubit 2.0 fluorometer.

    Article Title: Purinergic receptors play a key role in shock wave-induced proliferation
    Article Snippet: .. Successfully edited amplicons were purified using Wizard ® SV Gel and PCR Clean-Up Kit (Promega) and submitted for Sanger sequencing (Microsynth; primers in Table ). .. Editing efficiency was determined using inference of CRISPR edits (ICE) online analysis tool (Synthego Performance Analysis, ICE Analysis.

    Article Title: Whole genome characterization of Torque teno sus virus 1 (TTSuV1) in wild and domestic pigs: insights into genetic classification, host differentiation, and intra-host variation
    Article Snippet: .. Reaction conditions began with a 94°C denaturation for 1 min followed by 35 cycles of 94°C for 2 min, 62°C for 30 s, 72°C for 3 min, and a final extension at 72°C for 10 min. All products were purified using a Wizard SV Gel and PCR clean-up kit (Promega, Madison, WI, USA). ..

    Article Title: Association of placental mitochondrial DNA mutations on infant negative affectivity: modifying effects of maternal lifetime stress and infant sex.
    Article Snippet: Placenta DNA was extracted using the Promega Wizard Genomic DNA Purification Kit (Promega – Madison, WI, USA) and mtDNA was amplified using long range PCR. .. DNA clean-up was performed using a Wizard SV Gel and PCR Clean-Up kit (Promega). .. After mtDNA amplification, approximately 100 ng of DNA was used as input for library preparation using the NEBNext Ultra II FS DNA Library Prep kit (NEB, Ipswich, MA).

    Agarose Gel Electrophoresis:

    Article Title: Barcoded Rabies In Situ Connectomics for high-throughput reconstruction of neural circuits
    Article Snippet: .. To size-select the resultant linear products, the PCR reaction was loaded on an agarose gel, and gel extraction was performed with a Wizard SV Gel and PCR Clean-Up kit (Promega, A9281). .. Secondary purification was then carried out with DNA Clean & Concentrator-5 columns (Zymo Research, D4004).

    Gel Extraction:

    Article Title: Barcoded Rabies In Situ Connectomics for high-throughput reconstruction of neural circuits
    Article Snippet: .. To size-select the resultant linear products, the PCR reaction was loaded on an agarose gel, and gel extraction was performed with a Wizard SV Gel and PCR Clean-Up kit (Promega, A9281). .. Secondary purification was then carried out with DNA Clean & Concentrator-5 columns (Zymo Research, D4004).

    Plasmid Preparation:

    Article Title: Barcoded Rabies In Situ Connectomics for high-throughput reconstruction of neural circuits
    Article Snippet: Thermocycling conditions were likewise the same as the Illumina adaptor PCR, but with a fixed number of 3 cycles. .. The indexed viral and plasmid amplicons were then gel-purified with a Wizard SV Gel and PCR Clean-Up kit (Promega, A9281) and eluted with 25 μl water. .. The concentration and size distribution of these amplicon libraries were quantified with a High Sensitivity D1000 ScreenTape kit (Agilent, 5067-5584) on an Agilent 2200 TapeStation system.

    Purification:

    Article Title: Metagenomic and meta-metabolomic analysis of traditional Korean rice vinegar productions shows a large variability between producers.
    Article Snippet: For the fungal communities, the internal transcribed spacer 1 (ITS1) region of the rRNA transcribed unit was amplified by PCR using the primers BITS/B58S3 (Bokulich and Mills, 2013), extended with an Illumina-specific 5′ tag, as described previously (Vermote et al., 2018). .. The V4 and ITS1 amplicons obtained were purified using a Wizard SV gel and PCR clean-up kit (Promega, Madison, WI, USA) and size selected using AMPure XP magnetic beads (Beckman Coulter, Brea, CA, USA), according to the manufacturers’ instructions. .. The amplicon size distribution was determined with capillary electrophoresis using a Bioanalyzer 2100 with a High Sensitivity DNA Kit (Agilent, Santa Clara, CA, USA), and the concentrations were measured with a Qubit 2.0 fluorometer.

    Article Title: Purinergic receptors play a key role in shock wave-induced proliferation
    Article Snippet: .. Successfully edited amplicons were purified using Wizard ® SV Gel and PCR Clean-Up Kit (Promega) and submitted for Sanger sequencing (Microsynth; primers in Table ). .. Editing efficiency was determined using inference of CRISPR edits (ICE) online analysis tool (Synthego Performance Analysis, ICE Analysis.

    Article Title: Whole genome characterization of Torque teno sus virus 1 (TTSuV1) in wild and domestic pigs: insights into genetic classification, host differentiation, and intra-host variation
    Article Snippet: .. Reaction conditions began with a 94°C denaturation for 1 min followed by 35 cycles of 94°C for 2 min, 62°C for 30 s, 72°C for 3 min, and a final extension at 72°C for 10 min. All products were purified using a Wizard SV Gel and PCR clean-up kit (Promega, Madison, WI, USA). ..

    Magnetic Beads:

    Article Title: Metagenomic and meta-metabolomic analysis of traditional Korean rice vinegar productions shows a large variability between producers.
    Article Snippet: For the fungal communities, the internal transcribed spacer 1 (ITS1) region of the rRNA transcribed unit was amplified by PCR using the primers BITS/B58S3 (Bokulich and Mills, 2013), extended with an Illumina-specific 5′ tag, as described previously (Vermote et al., 2018). .. The V4 and ITS1 amplicons obtained were purified using a Wizard SV gel and PCR clean-up kit (Promega, Madison, WI, USA) and size selected using AMPure XP magnetic beads (Beckman Coulter, Brea, CA, USA), according to the manufacturers’ instructions. .. The amplicon size distribution was determined with capillary electrophoresis using a Bioanalyzer 2100 with a High Sensitivity DNA Kit (Agilent, Santa Clara, CA, USA), and the concentrations were measured with a Qubit 2.0 fluorometer.

    Sequencing:

    Article Title: Purinergic receptors play a key role in shock wave-induced proliferation
    Article Snippet: .. Successfully edited amplicons were purified using Wizard ® SV Gel and PCR Clean-Up Kit (Promega) and submitted for Sanger sequencing (Microsynth; primers in Table ). .. Editing efficiency was determined using inference of CRISPR edits (ICE) online analysis tool (Synthego Performance Analysis, ICE Analysis.



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